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KMID : 1101420180500020177
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Korean Journal of Clinical Laboratory Science
2018 Volume.50 No. 2 p.177 ~ p.182
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Inhibitory Effects of Zerumbone on MCP-1-Induced THP-1 Migration
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Kim Sa-Hyun
Kim Si-Hyun
Yu Sung-Ryul
Lee Pyeong-Jae
Moon Cheol
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Abstract
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This study examined the effects of zerumbone on monocyte migration. Monocytes are recognized as important mediators of various inflammatory diseases, and the possibility of controlling inflammatory diseases by regulating the monocyte functions, such as activity and mobility, has been reported. MCP-1, which is a chemokine with levels that increase upon inflammation, causes the migration of the monocyte cell line, THP-1. Migration occurred at a concentration of 10 ng/mL MCP-1, and the highest migration occurred at 100 ng/mL and 200 ng/mL. MCP-1-induced THP-1 migration decreased by more than 40% in the presence of zerumbone. The concentration of cAMP, an important secondary messenger of the CCR2 signaling pathway, the MCP-1 receptor, was increased in the culture medium after a zerumbone treatment. The concentrations of cAMP decreased significantly under the MCP-1 treatment condition only. On the other hand, an increase in cAMP was observed when zerumbone and MCP-1 were treated simultaneously. Erk phosphorylation induced by an MCP-1 treatment was also found to decrease with the zerumbone treatment. This study introduces the possibility of controlling inflammatory diseases through the function of zerumbone, which regulates the migration of monocytes.
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KEYWORD
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Migration, Monocyte, Monocyte chemoattractant protein 1, Zerumbone
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